After extensive testing of the fourth prototype, success was yielded. First of all, temperature validation was performed on my thermocycler using temperature sensors. This validation revealed that this prototype had a high degree of accuracy as well as temperature uniformity among the wells in the thermocycler. Secondly, a testing assay was performed to compare this thermocycler's accuracy with a conventional thermocycler's accuracy. Results showed a comparable level of accuracy between the thermocyclers, indicating that this thermocycler is capable of replacing laboratory grade thermocyclers without sacrificing a significant degree of accuracy. However, a more reproducible and compact version of the machine is desired, so the development of the 5th prototype is underway.
Figure 1 features the electrophoresis results from a conventional thermocycler.
Figure 2 features the electrophoresis results from my thermocycler.
Results: The bands produced from my thermocycler were comparable to those produced from the conventional thermocycler, although one column was missing due to loading errors preceding the electrophoresis test.
This figure features 3 lines that plot the temperatures of the three separate oil baths as they maintained their delegated temperature throughout PCR.
Results: Throughout the entire PCR test, the averaged temperature variation was +- .2 degrees Celsius throughout the wells in the thermocycler.