AUSTIN'S PROJECTS AND EXPERIENCES
Menu

Preliminary Validation of Low Cost, Low Power Detection System

8/22/2015

1 Comment

 
After constructing the circuit for the detection system with the raw electrical components, I sought preliminary validation of the detection system to determine the feasibility of this design.

Preliminary Testing Setup:
I first set up one light sensor with two LEDs and an optical filter to test one detection unit.  I then performed qPCR on 4 positive samples of DNA and 2 negative DNA samples. Afterwards, I performed two simple tests. First, I looked to see if the DNA could fluoresce. Secondly, I judged the specificity of the light sensor to determine if it is capable of detecting the small changes in the DNA's fluorescence.

Results:
Firstly, I determined that the DNA did indeed fluoresce when using the SYBR green fluorescent probe under a 480 nm LED. Secondly, I determined that the Cadmium Sulfide optical sensor could detect very small changes in the light intensity. This was accessed by observing a millivolt level of specificity when using this sensor in a voltage divider circuit under a variable light source. Lastly, I determined that I needed to purchase higher quality LEDs because the optical filters revealed that our current LEDs emit a range of wavelengths of light.

Fluorescing DNA:
Picture
1 Comment
resume writer provides the best executive resumes examples link
3/19/2018 03:23:31 am

Preliminary system check will allow you to avoid unnecessary problems and configure that everything works correctly.

Reply



Leave a Reply.

    Author

    Write something about yourself. No need to be fancy, just an overview.

    Archives

    August 2015
    July 2015
    April 2015
    March 2014
    February 2014

    Categories

    All

    RSS Feed

Proudly powered by Weebly
  • Background
  • 2015 Year
  • Overview
  • Trip To India- Blog
  • Thermocyler
  • About Me
  • Thermocycler Requirements
  • Sponsors
  • Links To My Project
  • Broadcom Competition Photos
  • Contact
  • Background
  • 2015 Year
  • Overview
  • Trip To India- Blog
  • Thermocyler
  • About Me
  • Thermocycler Requirements
  • Sponsors
  • Links To My Project
  • Broadcom Competition Photos
  • Contact