After constructing the circuit for the detection system with the raw electrical components, I sought preliminary validation of the detection system to determine the feasibility of this design.
Preliminary Testing Setup: I first set up one light sensor with two LEDs and an optical filter to test one detection unit. I then performed qPCR on 4 positive samples of DNA and 2 negative DNA samples. Afterwards, I performed two simple tests. First, I looked to see if the DNA could fluoresce. Secondly, I judged the specificity of the light sensor to determine if it is capable of detecting the small changes in the DNA's fluorescence. Results: Firstly, I determined that the DNA did indeed fluoresce when using the SYBR green fluorescent probe under a 480 nm LED. Secondly, I determined that the Cadmium Sulfide optical sensor could detect very small changes in the light intensity. This was accessed by observing a millivolt level of specificity when using this sensor in a voltage divider circuit under a variable light source. Lastly, I determined that I needed to purchase higher quality LEDs because the optical filters revealed that our current LEDs emit a range of wavelengths of light. Fluorescing DNA:
1 Comment
3/19/2018 03:23:31 am
Preliminary system check will allow you to avoid unnecessary problems and configure that everything works correctly.
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August 2015
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