Thermocycler REQUIREMENTS For Developing Nations:
One of the most difficult task to accomplish during the development of my thermocycler is the need to meet the machine's requirements for regions that lack money, energy, lab space, etc.
After reading a book by Dr. Eva Harris, Professor of Infectious Diseases and Vaccinology, I discovered their were many requirements!
- Inexpensive for wide distribution; developing nations pay high import taxes/shipping.
- Rugged, portable design. The parts must be easy to find and easily repaired locally.
- Easy to program temperatures and dwell times for annealing, extension and denaturation stages.
- Accurate temperatures and quick transition times to meet the required PCR protocols.
- Menu and manual in local language.
- Must operate without interruption during frequent power glitches or long interruptions
- Battery operation requires high efficiency (use tens of watts rather than the 400-800W lab model.